Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling.
نویسندگان
چکیده
Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.
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عنوان ژورنال:
- Chemical communications
دوره 49 41 شماره
صفحات -
تاریخ انتشار 2013